Figure 5. Expression of IFNγ receptors (IFNR1 and IFNR2) and effect of IFNγ stimulation on MHC class II (MHC-II) expression in undifferentiated hNSCs (cs23) and astrocytes differentiated from the same line.

(A) Expression of IFNR1 and IFNR2 in hNSCs, ADSCs and UC-MSCs assessed by RT-qPCR. (B) Fold changes in the expression of IFNR1 and IFNR2, and HLA-DRA and CIITA in astrocytes in relation to their parental hNSCs prior to differentiation assessed by RT-qPCR (Mean ± S.E.M; n = 3, biological replicates); *p < 0.05; **p < 0.01 (Student’s t-test). (C) Effect of IFNγ stimulation on hNSCs. Note the dose-dependent increase of MHC-II reactivity in hNSCs. (D) Effect of IFNγ stimulation on astrocyte; note MHC-II up-regulation upon IFNγ-treatment. (E) Negative control of the MHC-II staining (top panel) and stimulation of astrocytes or hNSCs with low MHC-II expression with hNSC conditioned medium (below); no MHC-II induction is observed. All scale bars are 100 μm.