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. 2016 Apr 15;6:24251. doi: 10.1038/srep24251

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Copyright © 2016, Macmillan Publishers Limited

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(A) Detection of MHC-II protein by immunocytochemistry in undifferentiated hNSCs and following astrocytic differentiation: differentiated astrocytes are MHC-II-negative and GFAP-positive. (B) Detection of MHC-II protein by immunocytochemistry in hNSCs from 3 different developing brains following neuronal differentiation for 3 weeks. Note the high expression of MHC-II protein in subsets of differentiated cells and increased expression upon differentiation. (C) Detection of MHC-II mRNA by RT-qPCR (values are means of 2 biological replicates) in the hNSC lines shown in (B); note that the MHC-II transcript increases in all lines upon differentiation. (D) Detection of MHC-II proteins by immunocytochemistry following differentiation of hNSCs (cs23) for 4 weeks using two different protocols; increased MHC-II expression is observed in both conditions. (E) Detection of HLA-DRA and CIITA mRNAs by RT-qPCR in hNSCs (cs23) under the differentiation conditions shown in (B). Mean ± S.E.M (n = 3, biological replicates; *p < 0.05; **p < 0.001 (one-way ANOVA followed by Tukey t-test).