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. 2016 Apr 15;6:24547. doi: 10.1038/srep24547

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Rats were sacrificed at 6 h after TBI. The brains of SHAM, TBI-Normothermia (NT) and TBI-Hypothermia (HT) were obtained. (A) Representative photographs of immunostaining for RIP1 and RIP3 in the cortex are shown (white arrows indicate immunoreactivity positive neurons). Original magnification ×100, magnification ×400 for interest area, the scale bars represented 200 μm and 50 μm respectively. (B) Statistical analysis of RIP1⁺ and RIP3⁺ neurons in the respective group (n = 6); Data are expressed as mean ± SD. *p < 0.05; **P < 0.01.