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. 2016 Apr 15;6:24566. doi: 10.1038/srep24566

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(a) The alteration of protein levels in the hippocampus after treatment with Ago299-5p (n = 5 per group). (b) The alteration of protein levels in cortex after treatment with Ago299-5p (n = 5 per group). (c) The fluorescence intensity of cleaved caspase-3 in the hippocampus was weakened with the decreasing intensity of Atg5 after treatment with Ago299-5p (n = 5 per group). Scale bar = 250 μm. (d) Apoptosis examination was performed by TUNEL assay, TUNEL positive cells were marked with arrows. Scale bar = 50 μm. Representative TUNEL assay images were captured from the boxed region. (e) The percentage of positive cells was calculated as the apoptosis rate (number of positive cells/total number of cells × 100%) (mean ± SD, n = 5 per group, *p < 0.05, ^#represents no significance).