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. 2015 Sep 9;116(11):2552–2562. doi: 10.1002/jcb.25200

Figure 5.

Figure 5

Effect of cholera toxin on the induction of EMT and secretion of IL‐6 in IHOK. (A) IHOK‐KGM cells were cultured in F‐medium (a, b, c), in F‐medium supplemented with 0.01 μg/ml of cholera toxin (d, e, f), and in F‐medium supplemented with 0.2 μg/ml of T3 (g, h, i) for 120 days, respectively. (B) Each group of cells was harvested, lysed, and immunoblotted for detection of EMT‐associated proteins (E‐cadherin, Vimentin, and snail) and proteins related to cell proliferation (Cyclin D1, p21). (C) Cells were incubated for 48 h in each culture medium, and each conditioned medium was analyzed for detection of secreted IL‐6. Data were presented as mean ± SD of three independent wells (*P < 0.05). (D) CT‐ treated IHOKs at the same passage were splitted into 4 dishes, and IL‐6 neutralizing antibody was added to each of the 3 dishes for one, two, or three days, respectively, at a concentration of 100 ng/ml. (E) Through western blot, the cells in the 4 dishes were analyzed for detection of EMT‐associated proteins.