Figure 2.

GADD45A does not induce cell death in HSCs and their progeny. (A): Occurrence of cell death events upon ectopic expression of GADD45A in LT‐HSCs was assessed by video‐microscopy and subsequent single cell tracking. Data were generated by tracking cells from two independent experiments. Only transduced cells (VENUS⁺) were included. (B): Experimental scheme and quantification of viability of donor‐derived cells in recipient bone marrow 3 weeks after transplantation determined via FACS. N = 6 mice per group. All data are represented as mean + SD. Abbreviations: BM, bone marrow; BMMNCs, bone marrow mononuclear cells; CTRL, control; FACS, fluorescence activated cell sorting; G45A, GADD45A; LT‐HSCs, long‐term repopulating hematopoietic stem cells.