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. 2015 Dec 10;231(6):1375–1384. doi: 10.1002/jcp.25259

Figure 1.

Figure 1

Kv1.2 clone and expression. Mouse K+ channel Kv1.2 was cloned into pcDNA3.1 plasmid and three stable Kv1.2 transfected COS‐7 cell lines were generated: Kv1.2‐A, Kv1.2‐B, and Kv1.2‐C. A,B: western blot showed different expression of Kv1.2 in three clones, and expression intensity analysis confirmed that Kv1.2‐C clone had the highest Kv1.2 expression in comparison to WT cells, Kv1.2‐A and Kv1.2‐B clones. C,D: quantitative PCR analysis of Kv1.2 expression in the three Kv1.2 clones confirmed the results in Western blot, comparing to two different house‐keeping genes GAPDH and HPRT, respectively. Results are presented as means ± SE; n ≥ 3. *P < 0.05.