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. 2015 Dec 22;34(3):756–767. doi: 10.1002/stem.2255

Figure 6.

Figure 6

Zoledronate (ZOL) enhances protection of mesenchymal progenitors from radiation induced damage in vivo. (A): Representative example of bone marrow (BM) from a mouse 24 hours after exposure to irradiation (3Gy) and stained for γH2AX foci. Dotted lines indicate endosteal surface. (B): Number of cells (identified by 4′,6‐diamidino‐2‐phenylindole staining) with more than five γH2AX DNA damage foci (in red) in the bone marrow (left panel) or adjacent to the cortical bone (right panel). (C): The number of BM colony forming unit fibroblast (CFU‐F) and CFU‐osteoblast (CFU‐O) following the same treatment. Data are expressed as mean ± SD and were analyzed by one way analysis of variance and Bonferroni post hoc test for multiple comparisons *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. Abbreviations: BM, bone marrow; CFU, colony forming unit; DAPI, 4′,6‐diamidino‐2‐phenylindole; IR, irradiation; PBS, phosphate buffer saline; UI, not irradiated; ZOL, zoledronate.