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. 2016 Apr 15;36(2):e00320. doi: 10.1042/BSR20160022

Table 1. Primers used for the amplifications of the T. bernacchii proteasome subunits cDNAs.

Primer SEQUENCE Tm (°C)
Alpha 4for 5′-GTAGTGGACCTCTTATTCTGTAGG-3′ 58.4
Alpha 4rev 5′-CAATACAGGATTTGGTGACAGG-3′ 58.1
Alpha 5for 5′-GACGCTTCCCCTGAAACAAG-3′ 60.6
Alpha 5rev 5′-GTGACATTCAGCCCAGGTG-3′ 60.1
Alpha 7for 5′-GGCTTCACAAATTGCTAACTAGC-3′ 59.5
Alpha 7rev 5′-CTCAAGTTATGATTTAGCTCTGCACA-3′ 60.2
Beta 1for 5′-CCATATTGCAGTGATACAGCGAG-3′ 60.5
Beta 1rev 5′-CTCAGTCCTTCCTCAGGGG-3′ 60.1
Beta 2for 5′-GTCGGGATACAGGGACCG-3′ 60.8
Beta 2rev 5′-AGCGGTCACTTGGCGC-3′ 62.8
Beta 3for 5′-CACAATAGCCAAGAAAAAGTGGAG-3′ 59.3
Beta 3rev 5′-GTTCGTGTGGTGATCTTGTCC-3′ 60.4
Beta 5for 5′-GGGAGTTTCAAAGATGGCTCT-3′ 59.2
Beta 5rev 5′-TTGTACTGCTGGTGCAGCAT-3′ 61.7