Figure 1.

Purity of the primary cultures of rat oligodendrocytes. Oligodendrocyte precursor cells were isolated from mixed glia cultures and cultured in Sato's medium + 0.05% FCS to induce differentiation. At day 1 of differentiation, more than 93% of the cells expressed the oligodendroglial lineage marker O4 (A) and at 5 days of differentiation, approximately 65% of the cells expressed myelin basic protein (MBP), a marker of mature oligodendrocytes (B). Scale bars, 50 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]