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. 2016 Apr 15;6:24578. doi: 10.1038/srep24578

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a)Examples of typical mitochondrial morphologies obsereved in mesothelial cell lines classified via fractal dimension and lacunarity. Maximum projection of Z-stack image series of TOM20 mitochondrial marker stain was used to quantify (b) mitochondrial fractal dimensions and (c) lacunarity. Images were converted to grayscale and analysed using the FracLac plugin from ImageJ. The entire mitochondria of each cell was analyzed. Data are presented as mean ± SEM, significance was calculated using an ANOVA with Tukey post test (*p < 0.05, **p < 0.005, ***p < 0.0001) n ≥ 40 for each cell type (c). (d) Profile of mesothelioma mitochondrial morphologies classified via fractal dimension and lacunarity.