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. 2016 Apr 15;6:24578. doi: 10.1038/srep24578

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Copyright © 2016, Macmillan Publishers Limited

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Mesothelioma and control cells in 1% serum media were treated with increasing concentrations of (a) Cisplatin, (b) Metformin, or (c) mdivi-1 as indicated for 72 hrs and cell viability was then assessed via Calcein-AM uptake as described. The results were normalized as a percentage of the untreated controls and analyzed via a sigmoidal dose response curve using Prism software (version 5.0) to calculate the EC50 value for each drug, n = 6 for each concentration shown ± the standard error of the mean.