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. 2016 Apr 15;36(2):e00316. doi: 10.1042/BSR20160027

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© 2016 The Author(s)

This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY).

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(A) Fluorescence images of HeLa cells transfected with ER-targeted roGFP-iL and its mutants were acquired (green) after fixation with 4% PFA. The cells were also co-stained with anti-GFP (red) and anti-Hsp47 (blue, ER marker). (B) Immunoblotting of ER-targeted roGFP-iL and its mutants with anti-GFP in 1% NP-40-soluble (S) and -insoluble (P) cell fractions.