Skip to main content
. 2016 Mar 30;15(4):405–419. doi: 10.1111/gbb.12287

Table 1.

Analysis of the differentiation of adult‐born granule cells. Quantitative analysis of co‐localization of BrdU, Tbr2 and NeuroD (marker combination 1) as well as BrdU, Calbindin and NeuN (marker combination 2) at 4 and 28 days after the initial BrdU injection. Combination 1: Type 1 (4 days after the initial injection) and mature neurons (28 days after the initial injection) (BrdU only); Type 2a (BrdU/Tbr2); Type 2b/3 (BrdU/Tbr2/NeuroD); immature neurons (BrdU/NeuroD). Combination 2: progenitor cells (BrdU only); immature neurons (BrdU and NeuN); mature neurons (BrdU, NeuN and Calbindin).

4 days after initial BrdU injection
Marker expression Corresponding stages Control (%) Mutant (%) P
BrdU+/Tbr2−/NeuroD− Type 1/(mature neurons) 39.02 ± 0.32  22.3 ± 5.43 0.019
BrdU+/Tbr2+/NeuroD− Type 2a n.c. 30.15 ± 2.66 34.61 ± 7.26 0.297
BrdU+/Tbr2+/NeuroD+ Type 2b/3 22.89 ± 2.33  42.33 ± 4.97 0.012
BrdU+/Tbr2−/NeuroD+ Immature neurons 7.94 ± 1.2   0.76 ± 0.76 0.004
BrdU+/NeuN−/Calbindin− Progenitor cells n.c. 34.55 ± 4.44 47.69 ± 8.7 0.125
BrdU+/NeuN+/Calbindin− Immature neurons n.c. 50.79 ± 2.91 43.34 ± 7.19 0.196
BrdU+/NeuN+/Calbindin+ Mature neurons 14.67 ± 1.53   8.97 ± 2.09 0.046
28 days after initial BrdU injection
Marker expression Corresponding stages Control (%) Mutant (%) P
BrdU+/Tbr2−/NeuroD− (Type 1)/mature neurons 94.44 ± 1.11 72.22 ± 6.19 0.012
BrdU+/Tbr2+/NeuroD− Type 2a  4.44 ± 1.11  1.11 ± 1.11 0.050
BrdU+/Tbr2+/NeuroD+ Type 2b/3  0 ± 0  16.67 ± 6.67 0.033
BrdU+/Tbr2−/NeuroD+ Immature neurons  1.11 ± 1.11  10 ± 0 0.001
BrdU+/NeuN−/Calbindin− Progenitor cells n.c    30 ± 5.77    40 ± 1.92 0.088 
BrdU+/NeuN+/Calbindin− Immature neurons 26.67 ± 6.67 54.44 ± 1.11 0.007
BrdU+/NeuN+/Calbindin+ Mature neurons 43.33 ± 3.33  5.56 ± 1.11 0.0002

Data are expressed in % of total number of BrdU‐positive cells counted; arrows indicate increase or decrease of parameters determined. P, P‐value; error bars, SEM; significance, P ≤ 0.05; n = 3. n.c., not changed.