Table 1.
Analysis of the differentiation of adult‐born granule cells. Quantitative analysis of co‐localization of BrdU, Tbr2 and NeuroD (marker combination 1) as well as BrdU, Calbindin and NeuN (marker combination 2) at 4 and 28 days after the initial BrdU injection. Combination 1: Type 1 (4 days after the initial injection) and mature neurons (28 days after the initial injection) (BrdU only); Type 2a (BrdU/Tbr2); Type 2b/3 (BrdU/Tbr2/NeuroD); immature neurons (BrdU/NeuroD). Combination 2: progenitor cells (BrdU only); immature neurons (BrdU and NeuN); mature neurons (BrdU, NeuN and Calbindin).
| 4 days after initial BrdU injection | |||||
|---|---|---|---|---|---|
| Marker expression | Corresponding stages | Control (%) | Mutant (%) | P | |
| BrdU+/Tbr2−/NeuroD− | Type 1/(mature neurons) | ↓ | 39.02 ± 0.32 | 22.3 ± 5.43 | 0.019 |
| BrdU+/Tbr2+/NeuroD− | Type 2a | n.c. | 30.15 ± 2.66 | 34.61 ± 7.26 | 0.297 |
| BrdU+/Tbr2+/NeuroD+ | Type 2b/3 | ↑ | 22.89 ± 2.33 | 42.33 ± 4.97 | 0.012 |
| BrdU+/Tbr2−/NeuroD+ | Immature neurons | ↓ | 7.94 ± 1.2 | 0.76 ± 0.76 | 0.004 |
| BrdU+/NeuN−/Calbindin− | Progenitor cells | n.c. | 34.55 ± 4.44 | 47.69 ± 8.7 | 0.125 |
| BrdU+/NeuN+/Calbindin− | Immature neurons | n.c. | 50.79 ± 2.91 | 43.34 ± 7.19 | 0.196 |
| BrdU+/NeuN+/Calbindin+ | Mature neurons | ↓ | 14.67 ± 1.53 | 8.97 ± 2.09 | 0.046 |
| 28 days after initial BrdU injection | |||||
|---|---|---|---|---|---|
| Marker expression | Corresponding stages | Control (%) | Mutant (%) | P | |
| BrdU+/Tbr2−/NeuroD− | (Type 1)/mature neurons | ↓ | 94.44 ± 1.11 | 72.22 ± 6.19 | 0.012 |
| BrdU+/Tbr2+/NeuroD− | Type 2a | ↓ | 4.44 ± 1.11 | 1.11 ± 1.11 | 0.050 |
| BrdU+/Tbr2+/NeuroD+ | Type 2b/3 | ↑ | 0 ± 0 | 16.67 ± 6.67 | 0.033 |
| BrdU+/Tbr2−/NeuroD+ | Immature neurons | ↑ | 1.11 ± 1.11 | 10 ± 0 | 0.001 |
| BrdU+/NeuN−/Calbindin− | Progenitor cells | n.c | 30 ± 5.77 | 40 ± 1.92 | 0.088 |
| BrdU+/NeuN+/Calbindin− | Immature neurons | ↑ | 26.67 ± 6.67 | 54.44 ± 1.11 | 0.007 |
| BrdU+/NeuN+/Calbindin+ | Mature neurons | ↓ | 43.33 ± 3.33 | 5.56 ± 1.11 | 0.0002 |
Data are expressed in % of total number of BrdU‐positive cells counted; arrows indicate increase or decrease of parameters determined. P, P‐value; error bars, SEM; significance, P ≤ 0.05; n = 3. n.c., not changed.