Figure 3.

FtsZ G196 substitutions are PC190723 resistant but 3‐MBA sensitive.

A. S. aureus strains ATCC 29213 (WT), R191P, G193D and G196A, and (B) B. subtilis strains 168 (WT) and DWA23 (FtsZ G196S) were streaked on TSA and nutrient agar (NA) respectively, in the absence and presence of either 3‐MBA (10 mM) or PC190723 (8 μg ml⁻¹), as indicated. Note that the same TSA and PC plates are also shown in Fig. 2A.

C. S. aureus strain G196A was grown in TSB at 37°C in the presence of either DMSO (1% v/v) or a series of dilutions of 3‐MBA (mM; as indicated). The optical density at 600 nm was measured at 6 min intervals and the mean of six replicates plotted against time.

D. Strain DWA27 (ftsZ cat[FtsZ G196S, N263K]) was streaked on NA + Cm, in the absence and presence of either PC190723 (8 μg ml⁻¹) or 3‐MBA (10 mM), as indicated. Plates were photographed after incubation at 37°C for 18 h.