Table 1.
Strain | Growth on solid media | Colony size | Cell morphology in liquid media | B. subtilis phenotype | |||
---|---|---|---|---|---|---|---|
TSA | + PC | +3MBA | DMSO | + PC | |||
WT | Yes | No | No | Normal | Normal | Balloons | – |
FtsZ R191P | No | Yes | No | Small | Balloons, lysis | Abnormal | Lethal |
FtsZ G193D | No | Yes | Yes | Small | Abnormal, lysis | Abnormal | Lethal |
FtsZ G196A | Yes | Yes | No | Normal | Normal | Normal | PC/8JR,b |
FtsZ V214F | Yes | Yes | Yes | Small | Abnormal | Abnormal | N.D. |
FtsZ N263K | Yes | Yes | Yes | Normal | Normal | Normal | BenzamideR |
FtsZ G266S | Yesa | Yes | Yes | Small | Abnormal | Abnormal | 3‐MBAR |
For reasons that we do not understand, strain G266S grew equally well in the absence and presence of PC on solid media but exhibited a dependent phenotype in liquid media.
This work (G196S) and Haydon et al. (2008) (G196A).
N.D., not determined; R, resistant.