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. 2015 Dec 22;99(5):925–944. doi: 10.1111/mmi.13276

Figure 5.

figure

HtrA from multiple H . pylori strains cleave E‐cadherin.

A. Domain structure of human E‐cadherin. Full‐length E‐cadherin (E‐cad‐FL) is a 130 kDa protein, which is composed of the 90 kDa extracellular domain amino‐terminal fragment (NTF) and 40 kDa carboxy‐terminal fragment (CTF‐1), containing the intracellular soluble 33 kDa CTF‐2 fragment.

B. In vitro cleavage assays of purified E‐cadherin with the various HtrAs. HtrA from all tested strains cleaved E‐cadherin, as monitored by the degradation of E‐cad‐FL and appearance of the cleaved 90 kDa NTF fragment (arrows).

C. Quantification of HtrA cleavage by the various HtrAs. Full cleavage of E‐cadherin was seen with recombinant HtrA of strain 26695 and was set 100% (last lane). Measurements were performed in triplicate.