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. 2015 Dec 22;99(5):925–944. doi: 10.1111/mmi.13276

Figure 6.

figure

Genetic complementation and expression of HP1020 and HP1021 genes in the operon downstream of htrA.

A. Schematic presentation of the complementation construct used to express the HP1020 and HP1021 genes (top) in the plasticity region of H . pylori (bottom). The expression construct at the top is composed of the htrA gene promotor followed by the HP1020 and HP1021 genes that are fused to HA‐ and FLAG‐tags, as indicated. At the 3′ end, a chloramphenicol resistance gene cassette was added to select clones.

B. This construct was transformed into H . pylori and chloramphenicol‐resistant colonies were screened for HA‐ and FLAG‐tagged proteins with the expected sizes of HP1020 and HP1021 proteins respectively. The corresponding wild‐type H . pylori strains did not reveal signals with these antibodies as expected. The α‐HtrA blot served as control, showing that equal amounts of protein are loaded in each lane.