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. 2015 Dec 22;99(5):925–944. doi: 10.1111/mmi.13276

Figure 7.

figure

Evidence for htrA representing an essential gene in H . pylori.

A. Structure of the recently developed HtrA inhibitor (Perna et al., 2015).

B. We used this HtrA inhibitor to study its effect on bacterial survival. Control experiments confirm that 50 μM inhibitor compound‐1 inhibits HtrA activity of H . pylori and C . jejuni in vitro using casein zymography of bacterial pellets. H . pylori and C . jejuni were grown in BHI medium in the presence or absence of different concentrations of the inhibitor as indicated and centrifuged. In the mock control, we added DMSO. After 12 h incubation, the cfu was determined for H . pylori on GC agar plates (C) and for C . jejuni on MH agar plates (D). The results show that inhibition of HtrA enzyme activity significantly inhibits the growth of H . pylori but not C . jejuni. The experiments were done in triplicate.