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. 2015 Mar 23;75(10):1051–1062. doi: 10.1002/pros.22989

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© 2015 The Authors. The Prostate, published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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IHC staining and FISH for ERG rearrangement and RNA‐ISH for ETV1. Panel A, whole section view of a prostate carcinoma tissue (Gleason score 3 + 3) showing ERG expression by IHC staining (panel on left). Right panel showing IHC staining for ERG over‐expression (×20 magnification) with the corresponding H&E stained sections (top). Bottom panel shows FISH for ERG genetic rearrangement using ERG break‐apart probes. Yellow arrow shows rearranged ERG, and co‐localizing red‐green signal depicts intact ERG locus. Panel B shows ETV1 expression by RNA‐ISH with a maximum intensity score 4 (×20 magnification).