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. 2015 Dec 28;231(6):1392–1404. doi: 10.1002/jcp.25282

Figure 5.

Figure 5

Western blotting of E11 protein (∼38 kDa) expression in MLO‐A5 cells treated with ± 10 μM of (A) MG132 (B) Bortezomib for 24 h prior to each time point over a 14 day culture period. β‐actin was used as a loading control. (C) Proteasome activity in MLO‐A5 cells over a 14 day culture period. Data are represented as mean ± S.E.M of three individual cultures. *P < 0.05, **P < 0.01 in comparison to day 0. (D) Western blotting of PSMB5 (∼25 kDa) expression in MLO‐A5 cells over a 14 day culture period. β‐actin was used as a loading control. (E) Immunoprecipitation of all ubiquitinylated proteins identified E11 to be expressed at both days 0 and 7 of MLO‐A5 cell culture, both ± addition of 10 μM MG132/Bortezomib. Data are represented as mean ± S.E.M.