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. 2015 Jul 29;33(10):3006–3016. doi: 10.1002/stem.2095

Figure 4.

Figure 4

STAT3 as a central player in graft‐induced reactive gliosis ex vivo but not in vivo. (A, B): Immunostaining showing GFAP overexpression and STAT3 phosphorylation in retinal explants cocultured with MSC at 4 days ex vivo. (C): Immunostaining showing the effect of S31–201 in suppressing GFAP expression (i–iii), increasing MSC engraftment (ii, vi), and inhibiting STAT3 phosphorylation (iv, v). (D): Immunostaining showing that GFAP overexpression induced by donor MSCs is suppressed in GFAP‐STAT3 cKO mice ex vivo (i–iv) but not in vivo (vi–viii); n = 3 per group. Scale bar = 100 µm. Error bars represent SEM, *, p < .05; **, p < .01. Abbreviations: GFAP, glial fibrillary acidic protein; GFP, green fluorescent protein; INL, inner nuclear layer; MSC, mesenchymal stem cell; ONL, outer nuclear layer; PBS, phosphate buffered saline; RGCL, retinal ganglion cell layer.