Figure 1.

Establishment of “small cell‐ness” features for lung carcinoma cell lines analyzing markers, mutations and morphology. NSCLC‐AdC (black), LCNEC (blue) and SCLC (green) were compared. NE marker expressions of (a) Chromogranin A (CHGA), (b) Synaptophysin (SYN), (c) CD56 and (d) ASCL1 were determined by qRT‐PCR and calculated by ΔΔCT‐method. (e) RB protein expression determined by Western Blot. (f) Mutations identified by amplicon‐based NGS. Allelic fraction was listed in %. (g) Cell morphologies determined in monolayer cell culture by microscopy. Bars indicate 100 μm. Cell size determined by forward scatter (FSC‐A) properties measured by flow cytometry.