Indices of oxidative stress and antioxidant defences in hearts from normoxic or hypoxic chick embryos with or without melatonin treatment at day 19 of incubation. Values are mean ± S.E.M. at day 19 of the expression of 3‐nitrotyrosine (A), the expression of 4‐hydroxynonenal (B), total nitrate and nitrite concentration (C), the expression of superoxide dismutase (D), catalase activity (E), and the expression of glutathione peroxidase (F) in the hearts of chick embryos incubated in either normoxia (N, n = 9), hypoxia (H, n = 10), hypoxia with melatonin (HM, n = 10), or normoxia with melatonin (NM, n = 10). n = 6 for all groups for (F). Significant (P < 0.05) differences are as follows: * versus N. † H versus HM (one‐ or two‐way ANOVA with Tukey and Bonferroni post hoc test, respectively).