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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: Biotechniques. 2015 Sep 1;59(3):137–148. doi: 10.2144/000114331

Figure 3. Expression of subventricular zone (SVZ) cell markers.

Figure 3

(A) SVZ markers expressed in GFP positive cells. (C) SVZ markers expressed in the GFP negative population. Markers of stem cells are defined as B type, transit-amplifying cells correspond to C type, neuroblasts correspond to A type; astrocytes correspond to As typ; and ependymal cells correspond to E type. Each cell type is identified by a combination of these markers (33). Expression of certain cell surface molecules can ensure the isolation of distinct cell types from GFP positive (B) and GFP negative (D) cell populations. Genes presented in italics were recently assigned as B cell markers using microarray data from the stem cell–enriched population (34). Heat maps reflect real-time PCR-derived Cq values for each transcript. Samples without amplification (w/o) were used as positive controls. Total RNA input of unamplified pooled SVZ cells or embryonic cells after the RT reaction corresponds to 3 μg. Cell #14 was excluded for technical reasons (low expression of ACTB and all other genes). Primer sequences are provided in Supplementary Table S4.