Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 2;68(3):242–251. doi: 10.1002/iub.1480

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Authors IUBMB Life published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

FGFR‐independent anti‐apoptotic activity of exogenous FGF1. NIH3T3 cells were serum starved for 24 h and then treated for 6 h with FGF1 (200 ng/mL) and heparin (10 U/mL) in the presence of the specific FGFR tyrosine kinase inhibitor, 100 nM PD173084. Apoptosis was measured using ApoLive‐Glo Multiplex Assay (Promega) Next, caspase‐3/7 activity, reflecting the apoptosis progression, along with cell viability were measured using ApoLive‐Glo Multiplex Assay (Promega). Caspase‐3/7 activity data were divided by cell viability values, then normalized toward the cells untreated with FGF1 (control), and denoted as relative caspase‐3/7 activity. Graphs represent the mean ± SD of four independent experiments (***P < 0.001).