Table 3.
Validation of 17 high-impact SNPs in 337 fertile stallions
| HWE | |||||
|---|---|---|---|---|---|
| Polymorphism | Candidate gene | HET | X 2 | DF | P-HWE |
| g.26775767G>C | NEURL | 0.116 | 22.094 | 1 | <0.0001 |
| g.77472655G>C | KDR | 0.228 | 0.324 | 1 | 0.569 |
| g.74610774C>T | CFTR | 0.004 | 0.001 | 1 | 0.976 |
| g.56937215C>T | OVGP1 | 0.111 | 0.810 | 1 | 0.368 |
| g.45985131A>G | FBXO43 | 0.032 | 0.082 | 1 | 0.775 |
| g.82699661C>T | TSSK6 | 0.068 | 0.380 | 1 | 0.538 |
| g.7083659A>T | SLC9A3R1 | 0.398 | 0.476 | 1 | 0.490 |
| g.40694339G>A | PKD1 | 0.013 | 0.013 | 1 | 0.911 |
| g.6704968C>T | GHRL | 0.019 | 18.572 | 1 | <0.001 |
| g.19034281C>T | FOXP1 | 0.104 | 0.959 | 1 | 0.327 |
| g.32635273T>C | BTNL2 | 0.168 | 120.250 | 1 | <0.0001 |
| g.35255390T>C | TCP11 | 0.022 | 0.040 | 1 | 0.842 |
| g.4323852G>A | SPATA31E1 | 0.009 | 0.007 | 1 | 0.933 |
| g.37453246G>C | NOTCH1 | 0.023 | 0.040 | 1 | 0.841 |
| g.37455302G>A | NOTCH1 | 0.226 | 0.272 | 1 | 0.602 |
| g.79813487A>T | ENSECAG00000020135 | 0 | 0 | 0 | – |
| g.25184403G>C | ENSECAG00000021286 | 0 | 0 | 0 | – |
For each polymorphism, corresponding candidate gene, heterozygosity (HET), test for Hardy Weinberg equilibrium (HWE) with the respective chi square (X 2) statistic, degrees of freedom (DF) and P-value (P-HWE) are given