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. Author manuscript; available in PMC: 2017 Apr 12.
Published in final edited form as: Cell Metab. 2016 Apr 12;23(4):610–621. doi: 10.1016/j.cmet.2016.03.007

Figure 1. Nobiletin (NOB) enhances amplitude of circadian rhythms.

Figure 1

(A) Nobiletin (NOB) chemical structure. (B) Primary screening of the NIH Clinical Collection (left) and Microsource Spectrum Collection (right) showing enhancement of the PER2::Luc ClockΔ19/+ reporter rhythm by NOB. (C) Left: Dose-dependent effects of NOB on reporter rhythms from PER2::LucSV cells. Right: Quantification of amplitude response to NOB doses. (D) NOB was not able to rescue reporter rhythms in PER2::Luc ClockΔ19/Δ19 fibroblast cells. (E) Clock-enhancing effects of NOB in pituitary explants from PER2::Luc WT (left) and PER2::Luc ClockΔ19/+ (right) reporter mice. (F) Left: Actograms illustrating the effect of vehicle or NOB on circadian behavior in RC-fed WT mice (n=5). Middle: Average wave plots summarizing wheel-running activity during 12–14 days of LD (12hr light, 12hr dark) or DD (Constant darkness) for indicated genotypes (n=5). Right: Daily total wheel-running activity during L:D or D:D conditions for the indicated genotypes (n=5). Data are represented as mean ± SEM.