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. 2016 Mar 16;6:101–107. doi: 10.1016/j.bbrep.2016.03.006

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Protein abundance in mitochondrial suspensions isolated without and with nagarse. Protein abundance detected by SDS-PAGE/Coomassie Blue (A), HPLC-ESI-MS/MS quantitative proteomics analysis to determine peptide ion abundance of mitochondrial and non-mitochondrial proteins in MITO+ and MITO− samples (B), and protein ratio of mitochondrial-to-non-mitochondrial protein in the same samples (C). Values are means±SE; MITO+, mitochondria samples treated with nagarse; MITO−, mitochondria samples not treated with nagarse; *P<0.05 between MITO+ and MITO-.