Table 2.
Total yield of citrate synthase and respiratory activity of mitochondria isolated without and with nagarse.
| MITO− | MITO+ | MITO+/MITO- (Fold Δ) | P Value | |
|---|---|---|---|---|
| Citrate synthase activity nmol min−1 | 329.3±54.1 | 366.7±53.0 | 1.11 | 0.11 |
| State 3 JO nmol min−1 | 31.1±5.7 | 42.5±7.5 | 1.37 | 0.01 |
| State 4 JO nmol min−1 | 9.1±1.8 | 9.1±1.3 | 1.00 | 0.98 |
| State 3 JPnmol min−1 | 153.5±27.5 | 229.5±38.8 | 1.49 | 0.01 |
Values are means±SE; MITO+, mitochondria samples treated with nagarse; MITO−, mitochondria samples not treated with nagarse; total yield of activity was calculated as the product of specific activity times the total protein yield (see mean values in Table 1); state 3 Jo, state 3 O2 consumption rate; state 4 Jo, state 4 O2 consumption rate; state 3 Jp, state 3 ATP production rate.