Figure 2.

Changes in the fluorescence of bovine serum albumin or lactalbumin + D-ribose treated with aqueous extracts from plant seeds. Bovine serum albumin or lactalbumin (final concentration 10 mg/mL) in the presence of D-ribose (final concentration 1M) was kept at 37°C in Tris-HCl buffer (pH 7.4). Aqueous extracts from plant seeds (4 μL: Blue columns and 8 μL: Yellow columns) was mixed with samples of bovine serum albumin (a) or lactalbumin, (b) +D-ribose for up to 10 days. The fluorescence intensity of glycation was recorded (λex 360 nm; λem 465 nm). Bovine serum albumin (or lactalbumin) and D-ribose were used as a control. Aliquots were taken for measurements of fluorescence spectra (λex = 360 nm). Values are the mean ± standard deviation of the three measurements. **P < 0.01, *P < 0.05 compared with the controls