Skip to main content
. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: Adv Exp Med Biol. 2014;801:593–600. doi: 10.1007/978-1-4614-3209-8_75

Fig. 75.2.

Fig. 75.2

Enzyme-mediated degradation and photo-degradation of A2E impacts proteasome activity in RPE cells. HRP was delivered to A2E-containing RPE by BioPorter vehicle. a–c HRP-mediated degradation of A2E followed by measurement of chymotrypsin- (a) and trypsin-like (b) proteasome activity. (c). HPLC quantitation of A2E confirms A2E degradation by HRP. Controls include absence of A2E; BioPorter in absence of HRP. d–e Photodegradation (430 nm light) of A2E followed by measurement of chymotrypsin- (d) and trypsin-like (e) proteasome activity. f HPLC quantitation of A2E and A2E isomers confirms A2E photodegradation. Controls include absence of A2E and A2E in absence of 430 nm exposure. Values normalized to untreated cells; mean ± SEM, 3 experiments; one-way ANOVA and Newman Keul Multiple Comparison test