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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: Cancer Res. 2015 Jun 29;75(16):3255–3267. doi: 10.1158/0008-5472.CAN-15-0325

Figure 5.

Figure 5

Cytoskeletal recovery in endothelial cells is enhanced by pre-conditioning heat exposure. (a) Experimental time courses for single heat exposure (1X heat) and re-exposure to heat (2X heat). (b) Representative fluorescence images of endothelial cell (EC) F-Actin filaments during recovery after final heating. Images depict cytoskeletal collapse and subsequent recovery. For each regimen, the same cell is shown across multiple time points to demonstrate the kinetics of EC recovery. (c) Relative EC viability for cells in 1X heat and 2X heat groups prior to and at different time points after heating. (n = 5 per group, *P<0.05 one-way ANOVA and Tukey's post-tests.) (d) Percentage of cells with visible membrane-spanning F-Actin filaments per field during the post-heating recovery period. (n = 1,072 – 1,213 cells from 6-8 fields of view per condition per time point, ****P<0.0001, two-way ANOVA and Bonferroni's post-tests.) Error bars, SE.