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. Author manuscript; available in PMC: 2017 Feb 27.
Published in final edited form as: J Mol Biol. 2016 Feb 17;428(5 Pt A):748–757. doi: 10.1016/j.jmb.2015.11.013

Figure 3. A case study for RNA design – Kyurem 7.

Figure 3

[A] A near-miss sequence design for Kyurem 7 (see Figure 2B) was designed by a player-created bot and misfolds only in one stem (red). [B] A successful solution with only afew base changes (green). [C] A slight variation in the Kyurem 7 target secondary structure has only minor changes in the lengths of the multiloops, but is much easier to solve due to the availability of low-energy designs for the expanded multiloops. Nucleotides are colored by base, with A in yellow, U in blue, G in red and C in green. Minimum free energy structures and loop energies (in kcal/mol) are based on the Turner 1999 parameters.