FIG. 6.

Sum of the extracted ion chromatograms (m/z 285, corresponding to the protonated β-hydroxylated metabolite; m/z 563, corresponding to the protonated protoporphyrin IX; m/z 616, corresponding to the protonated heme; m/z 845, corresponding to the protonated adduct between protoporphyrin IX and the β-hydroxylated metabolite) of liver samples after 4-week oral dosing with compound 1 at 200 mg/kg/day for one female treated dog (A) and one female control dog (B). Liver samples were collected at necropsy, extracted, and analyzed by UPLC-MS. The chromatograms were calculated with 50 mDa mass tolerance. The extracted ion chromatograms are displayed on the same intensity scale with the time window between 4 and 12 min as a ×6 zoom in.