FIG. 6.

Cadmium had no effect on the protein levels of KDM5A and KDM3A in BEAS-2B cells. BEAS-2B cells were treated with cadmium at 0, 0.5, 1.0, and 2.0 μM for 4 weeks (A) and 8 weeks (B). Whole cell lysates were extracted and then subjected to Western blot with antibodies against KDM5A or KDM3A. β-actin was used as a protein loading control. Three independent experiments were performed and representative results are shown. The relative intensities of the bands were quantified using Image J software (shown below the bands) and were expressed as the means ± SD in histograms (C, D).