FIG. 6.

Hg reduces the enzymatic activity of NEP. SH-SY5Y cells (1.5 × 10⁶) were differentiated with RA for 7 days, and then treated with 10 or 20 µM of Hg or 50 µM Pb for 48 h. A, A kinetic evaluation of NEP activity. B, The area under the curve from each treatment is presented. Data represent the mean ± SE from 3 independent experiments. Phosphoramidon was used as a positive control of NEP activity inhibition. *P ≤ .05 different with respect to control cells according to 1-way ANOVA and Tukey’s post-hoc tests. NEP=neprilysin. Phospho = phosphoramidon.