FIG. 8.

Hg changes the conformational structure of hrNEP. Recombinant hrNEP (0.5 µM in 50 µl of PBS pH 7) was used for titration with 2–20 molar equivalents of Hg or Pb dissolved in the same buffer. The spectrum was recorded every 2 nm in a CD spectrophotometer. Changes in the conformational structure of hrNEP induced by the addition of molar equivalents of Hg (A) or Pb (B) are presented. C, Effect of titration of molar equivalents of Hg or Pb at 222 nm in the hrNEP spectrum. The data were normalized to 1 when the rhNEP was free of any metal.