FIG. 6.

Functional activity of MRP2 in HepaRG cells and HHs. MRP2 activity was estimated using CDFDA in HepaRG cells and 4–5 days SCHHs and CCHHs. Efflux of fluorescent CDF, a substrate of MRP2, characterized by accumulation of fluorescence into bile canaliculi, was evaluated in standard and Ca²⁺- and Mg²⁺-free buffer in the presence and absence of MK571, an inhibitor of MRP2. Imaging was done using inverted microscope Zeiss Axiovert 200 M and AxioCam MRm.