Table. Plasmid DNA (in 50% ethanol): Variable starting concentrations ( Figure 1– Figure 4).
Sr No | Starting
Concentration |
Cleavage/Degradation | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
R | Cu | 1:1 | 1:0.2 | 1:0.1 | 1:0.02 | 1:0.01 | 1:0.002 | 1:0.001 | 1:0.0002 | ||
1 | 100µM: 100µM | - | - | ✔ | ✔ | ✔ | - | - | - | - | - |
2 | 500µM: 500µM | - | - | ✔ | ✔ | ✔ | ✔ | - | - | - | - |
3 | 1mM: 1mM | - | - | ✔ | ✔✔✔ | ✔✔✔ | ✔ | ✔ | - | - | - |
4 | 5mM: 5mM | - | - | ✔✔✔ | ✔✔✔ | ✔✔✔ | ✔✔✔ | ✔✔✔ | ✔ | ✔ | - |