Plasmid DNA (in various solvents): Starting concentration 5mM:5mM ( Figure 4; Figure 7– Figure 9).
Sr No | Solvent | Cleavage/Degradation | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
R | Cu | 1:1 | 1:0.2 | 1:0.1 | 1:0.02 | 1:0.01 | 1:0.002 | 1:0.001 | 1:0.0002 | ||
1 | 50% Ethanol | - | - | ✔✔✔ | ✔✔✔ | ✔✔✔ | ✔✔✔ | ✔✔✔ | ✔ | ✔ | - |
2 | 50% Acetonitrile | - | - | ✔ | ✔✔✔ | ✔✔✔ | ✔✔✔ | ✔✔✔ | ✔ | - | - |
3 | 3mM NaOH | - | - | ✔ | ✔ | ✔ | ✔✔✔ | ✔✔✔ | ✔ | ✔ | ✔ |
4 | Water | - | - | ✔ | ✔ | ✔ | ✔ | ✔ | ✔ | ✔ | - |