Fig. 3.

Phylogenetic analysis of bfPTH-like and bfPACAP/GLUCs peptide precursors with vertebrate PTH, GCG and PACAP families. Full-length precursors amino acid sequences from vertebrates anole lizard (an), chicken (ch), elephant shark (el), human (hu), mouse (mo), Xenopus (xe), zebrafish (ze), and invertebrate Branchiostoma floridae (bf) were used to generate a maximum likelihood tree using Mega 6.06 (with JTT model and combined with +I, +G, +F). One thousand bootstrap simulations were used to test the reliability of branching. Vertebrate PACAP, GCG, and PTH subfamilies are highlighted in different colors. Predicted mature peptides within bfPTH1, bfPTH2, and bfPACAP/GLUCs precursors are labeled next to the gene names. Number of sites used in this phylogenetic reconstruction is 57.