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. 2015 Apr 3;32(8):2048–2059. doi: 10.1093/molbev/msv087

Fig. 4.

Fig. 4.

cAMP response of bfPTHR-like receptors to various peptides. Intracellular cAMP accumulation in response to 100 nM amphioxus and vertebrate superfamily peptides was tested on CHO-K1 cells transiently transfected with (A) bf98C, (B) bf95, (C) bf98A, (D) bf98B, (E) bf173, and (F) pcDNA3.1, negative control. Peptide species: Branchiostoma floridae (bf), coelacanth (co), goldfish (gf), human (hu), sea lamprey (lp), and zebrafish (ze). Fold changes in cAMP were obtained by comparing with the control (no peptide treatment). The red lines represent the control value. Data are means ± SEM from at least three experiments performed in duplicate. All data were analyzed by one-way analysis of variance (ANOVA) and followed by Dunnett’s test using the computer software PRISM (GraphPad Software Inc., San Diego, CA). *, P ≤ 0.001 and #, P ≤ 0.05.