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VPA increases intrachromosomal recombination in embryonic head tissue. qRT-PCR analysis of lacZ expression in embryonic head (i), heart (ii), and trunk (iii) tissues following maternal administration of VPA (500 mg/kg) for 1, 3, 6, or 24 h. All data were quantified using the delta-delta Ct method utilizing hypoxanthine-guanine phosphoribosyltransferase as the housekeeping gene control. * indicates a significant difference from saline-treated controls (p < 0.05).
