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VPA alters transcript levels of Brca1 and Brca2 in embryonic tissues. qRT-PCR analysis of Brca1 (A) and Brca2 (B) expression in embryonic head (i), heart (ii), and trunk (iii) tissues following maternal administration of VPA (500 mg/kg) for 1, 3, 6, and 24 h. All data were quantified using the delta-delta Ct method utilizing hypoxanthine-guanine phosphoribosyltransferase as the housekeeping gene control. * indicates a significant difference from saline-treated controls (p < 0.05).
