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The effect of treatment with METH or 4-MMC, either alone or in combination with GBR12909, on (A) basal respiration and (B) maximum respiration. The assay was performed in a Seahorse Bioscience XFe96 analyzer. Basal and maximum mitochondrial respiration/OCR was calculated by subtracting baseline OCR and FCCP-induced maximum OCR from the non-mitochondrial OCR after injection with antimycin A + rotenone. *p < 0.05, **p < 0.01, ***p < 0.001. N ≥ 5 per group.
