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. 2014 Sep 12;141(1):278–291. doi: 10.1093/toxsci/kfu125

FIG. 8.

FIG. 8.

Exposure to DBP influences the fate of sperm in Drosophila. To determine the effect of DBP on the fate of sperm in the female milieu, each of the control or exposed Protamine B-EGFP male was presented with broods of three virgin females every 24 h for 3 days. The first mated female of brood1 (considered as mate I) of exposed male, had significantly fewer sperm in SR (seminal receptacle; A) at 2 h ASM whereas the sperm counts in SP (spermathecae; B) did not differ from those in mate I of control males. However, mate IV (from brood 2) and mate VII (from brood 3) of male exposed to 100μM DBP contained fewer sperms in both seminal receptacle (C and E) and spermathecae (D and F) when compared with those mates in control broods (*p < 0.05; **p < 0.01; N = 10–15/replicate). In addition, sperm in seminal receptacles (SR) from X mates of control (G) or exposed (H) Protamine B-EGFP males were counted and we observed significant reduction in the number of sperms in seminal receptacle of females mated to Protamine B-EGFP males, developmentally exposed to 100μM in comparison to control (*p < 0.05; N = 10–15; panel I). Panels (J) and (K) depict sperm in the spermathecae of X mates of control or exposed Protamine B-EGFP males, respectively. Panel (L) presents the number of sperm stored in spermathecae of females mated to exposed males, when compared with their controls (*p < 0.05; N = 10–15/replicate).