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. 2015 Oct 12;45(12):3386–3403. doi: 10.1002/eji.201545794

Figure 4.

Figure 4

IFN‐α/β induction is unaffected in Ddx60−/− MEF and BMMCs in response to the transfection of different RNA stimuli. (A) Levels of mIFN‐α in 24 h culture supernatants from Ddx60+/+, Ddx60+/−, and Ddx60−/− MEFs transfected with indicated amounts of IVT‐RNA, poly(I:C), short poly(I:C), and RNA isolated from EMCV infected Vero cells (Vero‐EMCV‐RNA). (B–E) Levels of mIFN‐α or mIFN‐β in 24 h culture supernatants from Ddx60+/+ and Ddx60−/− BMMCs transfected with different concentrations of (B) IVT‐RNA, (C) poly(dA:dT), (D) poly(I:C) or (E) Vero‐EMCV‐RNA. Water was used as a transfection control. The mean (±SD) of triplicate technical replicates is shown. ns refers to not significant. For all experiments one representative of three independent experimental repeats is shown.