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. 2015 Dec 8;46(2):381–389. doi: 10.1002/eji.201545858

Figure 5.

Figure 5

Coregulation of Clec4d and Mincle during pulmonary M. bovis BCG infection. CTLR expression on CD45+ pulmonary leukocytes in naïve wild type and Clec4d−/− mice and 48 h following infection with M. bovis BCG were analyzed by flow cytometry. (A) CD45+ and (B) population‐specific cellular expression levels of Clec4d were determined by flow cytometry. Shown are pooled data from two independent experiments, n = 6–9 mice per group from both experiments. (C) Representative histogram of Clec4d expression on alveolar macrophages following infection as above. Black dotted histogram, wild‐type naïve; gray filled histogram, Clec4d−/− +BCG; solid black histogram, wild‐type +BCG. (D) Expression levels of Mincle on CD45+ cells. Shown are pooled data from three experiments, n = 9–15 mice per group. (E) Expression levels of Dectin‐2 on CD45+ cells. Shown are pooled data from three experiments, n = 9–15 mice per group in total. (A, D, E) Each symbol represents an individual mouse and bars represent mean + SEM. Statistical significance was determined by Student's t‐test, *p < 0.05, ***p < 0.001.