Figure 8.

The expression of membrane and nuclear progesterone receptors on CD8⁺ and CD4⁺ T cells. (A) PBMCs isolated from maternal and control donors were sorted into pure populations of CD8⁺ and CD4⁺ T cells and purity was checked (n = 4). (B) RNA was extracted from the CD8⁺ and CD4⁺ T‐cell populations, and real‐time PCR was used to determine the expression of the nuclear progesterone receptors (nPR), progestin and adipoQ receptor family members 5, 7, and 8 (PAQR5, 7, and 8), and the progesterone receptor membrane component members 1 and 2 (PGRMC1 and 2). Human uterus cDNA was used as a positive control and GAPDH as the endogenous control/housekeeper. Data shown as mean + SEM of n = 4 samples in each group. (C) Summary of nuclear and membrane progesterone receptor expression levels found on CD8⁺ and CD4⁺ T cells relative to the levels in uterus.